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D-36
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Cat.No.
CSC-C1076
Description
Established from the bone marrow of a DBA/2 mouse in 1986; cells can be used for an IL-10 assay
Species
Mouse
Source
mast cells
Recommended Medium
90% SuperCult® IMDM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1 ng/ml mouse IL-4 + 1 ng/ml mouse IL-3
Culture Properties
suspension
Morphology
growing in suspension in small clumps
Karyotype
Murine near-diploid karyotype with 2% tetraploidy - 40(37-41)<2n> - centric fusion only rarely present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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