Established from a metastatic lymph node of a 69-year-old man with colon carcinoma in 1984
Human hyperdiploid karyotype with 4% polyploidy - 57(52-58)<2n>XXY, +X, +3, +7, +8, +10, +11, +12, +15, -17, -18, +22, +4mar, add(3)(p11), amp(7)(q11.23)x2, del(12)(q21/22q24.2)x2 - trisomy 7 and 12 are associated with colon carcinoma
round or spindle-shaped adherent cells growing as monolayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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