Established from the ascites fluid of a 71-year-old man with colon carcinoma in 1975; The cell line was established from the same patient as the cell lines COLO 201 and COLO 205
Human hyperdiploid karyotype with 10% polyploidy - 72(66-77)<2n>XX, -Y, +1, +3, +3, +5, -6, +7, -8, +9, -10, -13, -18, +20, -21, +3mar, i(1q), der(1)t(1;18)(q11;p11), der(3) t(3;?)(p21;?)x2, del(10)(q23.3), der(12)t(3;12)(q11;p13) - a small supernumerary
mostly round cells; about 80% are adherent cells; about 2-8% spindle-shaped cells; some giant cells
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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