Established ca. 1986 from the hepatocellular carcinoma induced in vivo by chronic nutritional treatment of an adult male rat (Wistar AF-Han) with diethylnitrosamine
epitheloid cells growing adherently in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology:cytokeratin +Viruses:SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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