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CAL-54

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Cat.No.
CSC-C2548
Description
Established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987
Organism
Human
Karyotype
Human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma
Source
kidney carcinoma
Culture Properties
monolayer
Morphology
epitheloid, adherent cells growing in monolayers
Recommended Medium
85% DMEM +15% h.i.FBS + 0.4 μg/ml hydrocortisone + 10 ng/ml EGF
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CM-1010X
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