CAL-54

Cat.No.: CSC-C2548

Species: Homo sapiens (Human)

Source: Pleural Effusion Metastasis

Morphology: epitheloid, adherent cells growing in monolayers

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C2548

Description

Established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987

Species

Homo sapiens (Human)

Source

Pleural Effusion Metastasis

Recommended Medium

85% DMEM +15% h.i.FBS + 0.4 μg/ml hydrocortisone + 10 ng/ml EGF

Culture Properties

monolayer

Morphology

epitheloid, adherent cells growing in monolayers

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma

Disease

Renal Cell Carcinoma

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

CAL 54; CAL54; Centre Antoine Lacassagne-54

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Ask a Question

Write your own review

You May Also Need

CM-1010X

CS-001Y

TUHR4TKB

Description: Japanese renal carcinoma cells. Cell growth is slow.

Cat#: CSC-C6399J INQUIRY

View Product

TUHR14TKB

Description: Japanese renal carcinoma cells.

Cat#: CSC-C6381J INQUIRY

View Product

G-401

Description: Rhabdoid tumor of kidney (formerly classified as Wilms' tumor)

Cat#: CSC-C6937J INQUIRY

View Product

NH-12

Description: Human cell line derived from neuroblastoma.

Cat#: CSC-C6328J INQUIRY

View Product

SN12PM6

Description: Histopathology: carcinoma, renal cell

Cat#: CSC-C9612L INQUIRY

View Product

HEK 293

Description: The cells express the transforming gene of adenovirus 5. Adenovirus 5 DNA from both the right and left ends of the viral genome are present. The line is excellent for titrating human adenoviruses. ...

Cat#: CSC-6980J INQUIRY

View Product

For research use only. Not for any other purpose.