BxPC-3
Cat.No.: CSC-C9163W
Species: Homo sapiens (Human)
Source: Pancreas
Morphology: epithelial
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BxPC-3 is a well-characterized human pancreatic ductal adenocarcinoma (PDAC) cell line established from a primary pancreatic tumor of a 61-year-old female. It exhibits epithelial morphology, adherent growth, and robust expansion in standard culture. It is tumorigenic in immunodeficient mice and supports subcutaneous and orthotopic xenograft models, enabling in vitro and in vivo studies of pancreatic cancer biology.
A key advantage is that BxPC-3 is KRAS wild-type, distinguishing it from most PDAC lines and patient tumors, which frequently harbor KRAS mutations. This makes BxPC-3 a valuable model for the clinically distinct KRAS wild-type subset of pancreatic cancer and a useful comparator for KRAS-mutant lines such as Panc-1 and Mia PaCa-2. It is widely used to study tumor progression, chemoresistance, radiation response, cancer stem cell biology, and biomarker discovery, as well as for drug screening and target validation. Its well-characterized genomic and transcriptomic profiles, commercial availability, and reproducible growth support comparative and high-throughput studies. BxPC-3 is amenable to transfection, siRNA/CRISPR perturbation, flow cytometry, immunostaining, Western blot, qPCR, co-culture, and 3D models.
Role of Octamer Transcription Factor 4 in Proliferation, Migration, Drug Sensitivity, and Stemness Maintenance of Pancreatic Cancer Cells
To investigate the role of octamer transcription factor 4 (OCT4) in pancreatic cancer stem cells (CSCs) and its effect on pancreatic cancer (PC) cell proliferation, migration, drug sensitivity, and stemness maintenance.
BxPC-3 cells were used to assess the effects of OCT4 modulation on cellular behavior. Proliferation, migration, and stemness of BxPC-3 cells were evaluated, and the PI3K/AKT/mTOR pathway was examined to gain mechanistic insights.
OCT4 modulation altered BxPC-3 cell proliferation, invasion, and stemness, with OCT4 overexpression (OV-OCT4) enhancing these properties and OCT4 interference decreasing them. OV-OCT4 activated the PI3K/AKT/mTOR pathway, which correlated with an increase in PC stem cells (PCSC).
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