Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice
adherent epithelial cells growing in monolayers, but not reaching complete confluence (ca 80%)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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