Derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens
normal kidney cells
epithelial-like cells growing in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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