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Cat.No.
CSC-C6185X
Description
Cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-purified chicken retina E10 proteins; described as secreting a mouse monoclonal antibody (IgG) reacting with chicken retina (Müller glia cell-specific antigen)
Species
Mouse
Source
hybridoma (anti-chicken retina)
Recommended Medium
Culture Properties
suspension
Morphology
round cells growing singly or in small clumps in suspension, some cells are slightly adherent
Karyotype
Murine hypertetraploid karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Immunology: secretion of IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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