Sq-1979

Cat.No.: CSC-C6597J

Species: Mus musculus (Mouse)

Morphology: Epithelial-like

Culture Properties: Adherent

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Cat.No.

CSC-C6597J

Description

Metastatic epithelial cell

Species

Mus musculus (Mouse)

Recommended Medium

EMEM + 10% FBS

Culture Properties

Adherent

Morphology

Epithelial-like

Disease

Mouse Oral Cavity Squamous Cell Carcinoma

Storage and Shipping

Storage condition: Liuqid Nitrogen, -180°C
Shipping: Dry Ice, Frozen

Synonyms

Sq1979

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Sq-1979 Cells are mouse squamous cell carcinoma cells isolated from a tumor that originated from the oral cavity of a mouse. Sq-1979 cells are used as an in vitro model system for oral squamous cell carcinoma (OSCC). Given their epithelial heritage, Sq-1979 cells are likely to mirror key biological characteristics of oral squamous cell carcinomas. Sq-1979 cells can therefore be used as a model system to study mechanisms of oral tumor initiation, progression and therapeutic response in mice.

Sq-1979 cells typically grow as adherent cells with polygonal morphology in culture, which is typical of epithelial cells. The cells often grow as compact cell colonies with clearly defined cell-cell junctions. Sq-1979 cells express multiple squamous epithelial markers as well as carcinoma-associated markers, including cytokeratins (CK5/6 and CK14), E-cadherin and p63. In addition to epithelial markers, Sq-1979 cells can also express markers associated with epithelial-mesenchymal transition (EMT), such as vimentin and N-cadherin, and become highly motile under specific conditions. Sq-1979 cells have been used extensively to study oral cancer cell proliferation, differentiation, migration, invasion, tumor microenvironment and signaling. Sq-1979 cells are frequently used to study EGFR signaling in OSCC, MAPK signaling, NF-κB and inflammatory responses, as well as mechanisms of immune-related functions in oral tumors.

Antitumor Effect of Z-100 on Sq-1979 Tumor Model

The tumor microenvironment critically determines cancer immunotherapy efficacy, with "hot" tumors showing greater T-cell infiltration and better responses. Z-100, extracted from Mycobacterium tuberculosis Aoyama B strain, increases immune cell cytokine production. Horii et al. examined whether Z-100 can induce hot tumors and enhance antitumor immunity.

Z-100 has been examined in clinical trials for cervical squamous cell carcinoma, but no standard mouse cervical cancer cell line exists. Therefore, they used Sq-1979, a mouse oral squamous cell carcinoma cell line, for subcutaneous tumor modeling (Fig. 1a-c). Treatment with Z-100 dose-dependently suppressed tumor growth and tumor-bearing rate (Fig. 1d, e). A dosage of 1 mg/kg of Z-100 significantly prolonged survival (Fig. 1f) and decreased tumor size compared to controls. Here we only show comparison of Saline vs. 1 mg/kg Z-100 because of low sample number for the other groups. They also performed ATP assays in vitro and demonstrated that unlike positive control cisplatin, Z-100 did not directly decrease Sq-1979 cell viability (Fig. 2).

Antitumor effect of Z-100 in Sq-1979 model. — Fig. 1. Antitumor effect of Z-100 in Sq-1979 model (Horii T, Orikawa Y, *et al.*, 2022).

Direct effect of Z-100 on Sq-1979 cell line. — Fig. 2. Direct effect of Z-100 on Sq-1979 cell line (Horii T, Orikawa Y, *et al.*, 2022).

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