QualiNuclei® FFPE RNA Extraction Kit (96-Well)

CNEK-R1811H

QualiNuclei® FFPE RNA Extraction Kit (96-Well) provides a rapid method for high throughput isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to cross linking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. This kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. Purification can be performed by using either a vacuum manifold or centrifugation.

2 x 96-well plates

Reagents should remain stable for at least 1 year in their unopened containers.

All solutions should be kept tightly sealed and stored at room temperature.
The DNase I should be stored at -20°C upon arrival.
The Proteinase K should be stored in aliquots at -20°C upon reconstitution.

Room temperature or blue ice

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.