QualiNuclei® FFPE RNA/DNA Extraction Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. This kit conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. This kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.
Storage & Stability
Reagents should remain stable for at least 1 year in their unopened containers.
All solutions should be kept tightly sealed and stored at room temperature.
The DNAse I and Proteinase K should be stored at -20°C upon arrival.
Room temperature or blue ice
PCR reactions, Northern blotting, RNase protection and primer extension, expression array assays, sequencing, Southern blotting and SNP analysis.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.