The Immortalized Drosophila Embryonic Cells (R1) was established by serial passaging of primary cultures from Act5C > UAS-RasV12embryos. The RasV12immortalized cell lines have significantly up-regulated cell-cycle and cell-division gene expression including gene targets of the E2 promoter binding factor/retinoblastoma protein (E2F/RB) pathway. Specifically, R1 express upregulated Cyc A, stg, dap, cdc2c, tum, sti, pav, stg, dup, dap, Orc2, and Mcm2. The cell lines are in a proliferative undifferentiated progenitor-like state associated with increasing levels of Polycomb Group (PcG) transcripts during the immortalization process. Related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells, the immortalized R1 cell line retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. The cell line is recommended as a tool for Drosophila embryogenesis and muscle cell differentiation and proliferation.
Serial passaging of primary cultures from Act5C > UAS-RasV12embryos
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
For Research Use Only
1) Inducing muscle cell differentiation by treating cells with ecdysone and perform immunofluorescence staining and qPCR to assess the upregulated expression of mhc and Tropomyosin (Tm) .
2) Up-regulated genes in DPCs are analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery) .
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.