Human iPSC-derived Endothelial Cells

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Scientific Data
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Cat.No.

CSC-00857L

Description

Human iPSC-derived Endothelial Cells display typical morphology and markers, such as CD31. When thawed and maintained according to the instructions in the user guide, iPSC-derived endothelial cell cultures are viable long-term and are suitable for a variety of characterization assays.

Species

Human

Application

For Research Use Only

Shipping

Dry Ice

Storage and Shipping

Remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) are generated by directed differentiation of hiPSCs through mesodermal and endothelial lineages using sequential growth factors and small molecules. They express canonical endothelial markers—CD31/PECAM-1, VE-cadherin, VEGFR2/KDR, CD34, von Willebrand factor, and eNOS—and exhibit endothelial functions including acetylated LDL uptake, UEA-1 lectin binding, migration, proliferation, tube formation, nitric oxide production, and barrier formation measurable by TEER/permeability.

Their key advantage is human, patient-specific origin with unlimited scalability. Unlike primary endothelial cells such as HUVECs, iPSC-ECs provide a renewable source, reduce donor variability through clonal selection, and enable repeated and high-throughput experiments. Because they retain the donor genotype, they are ideal for modeling genetic vascular diseases, diabetes, pulmonary hypertension, and retinal vasculopathies, and for personalized drug-response testing. Paired with CRISPR/Cas9, isogenic controls can be generated to dissect disease mechanisms. They also support co-culture with iPSC-derived cardiomyocytes, pericytes, or organoids and are explored for regenerative cell therapy and tissue engineering.

Crosstalk Between CGRP and Piezo1 in Human iPSC-Derived Endothelial Cells Relevant to Migraine

It is becoming increasingly evident that the vasculature is implicated in migraine pathophysiology. Calcitonin gene-related peptide (CGRP) acts as one of the key migraine mediators through various mechanisms that includes endothelium-mediated cerebral vessel vasodilation. Endothelial cells express mechanosensitive Piezo1 channels and have been suggested to play a role in migraine pathophysiology. However, the crosstalk between these two migraine-related signaling pathways remains unclear.

We measured intracellular calcium (Ca²⁺) in human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs), after exposure to Yoda1, a specific Piezo1 channel agonist, with and without CGRP. In addition, we investigated the effects of CGRP and Yoda1 on cellular remodeling by staining for focal adhesion (FA) protein paxillin using immunocytochemistry.

Our data suggest that a one-hour sensitization of hiPSC-ECs with CGRP followed by application of Yoda1 leads to a higher intracellular Ca²⁺ level compared to when Yoda1 and CGRP are acutely applied separately or combined, suggesting at least indirect crosstalk between the two signaling pathways in the vascular system. CGRP receptor antagonist BIBN4096 significantly reduced the intracellular Ca²⁺ level under this sensitization protocol, confirming effective CGRP pathway blockade. The results also show that a one-hour sensitization of CGRP and Piezo1 activation affects cellular remodeling as evidenced by an increased number and area size of paxillin FA points per cell in hiPSC-ECs.

CGRP exposure for 1 hour sensitizes Piezo1 channels leading to a higher intracellular Ca2+ level in hiPSC-ECs. — Fig. 1. Application of Yoda1 and/or CGRP to induce intracellular Ca²⁺ levels in hiPSC-ECs (Gkouzioti, Vasiliki, *et al.*, 2025).

CGRP binding and Piezo1 channel activation promote cellular remodeling via paxillin focal adhesion point reorganization. — Fig. 2. Paxillin focal adhesion (FA) numbers and area size detection after Yoda1, CGRP, CGRP + Yoda1 and CGRP 1h_Yoda1 application in hiPSC-ECs (Gkouzioti, Vasiliki, *et al.*, 2025).

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For research use only. Not for any other purpose.