Rat Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of 1-week old Sprague??awley Rats. Rat Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.
Rat Bone Marrow Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.
Standard biochemical procedures performed include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Complete Mesenchymal Stem Cell Medium
Storage and Shipping
Creative Bioarray will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 ?C) until ready for experimental use. Live-cell shipment is also available on request.
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