Tumor induced in nude mouse injected with blast cells from ascites fluid of newborn Balb/c mouse injected intraperitoneally with Rauscher murine leukemia virus (supernate of producer cell line JLS-V9); descibed to be heterotransplantable, to form colonies in soft agar, to display myeloid cytochemical (myeloperoxidase) and functional (phagocytosis) features, and to differentiate terminally in response to DMSO
Murine hyperdiploid karyotype - 41(38-41)XY, structurally and numerically rearranged
spheroidal cells growing singly in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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