Established from transplantable tumors induced in syngeneic mice inoculated with cell suspensions prepared from lymph nodes infiltrated by lymphatic leukemia induced in 6-week-old DBA/2 mice by Rauscher virus (present in supernate of JLSV-9 cells); described as expressing T cell surface markers (Thy-1, T-200, Lyt-1), high constitutive TdT activity, and as differentiating along T-helper lineage in response to treatment with DMSO or phorbol ester TPA
Murine hyperdiploid karyotype - 42XX, +14, +15 - no centric fusion markers present - matches published karyotype
spheroidal cells growing singly in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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