RH-18

Cat.No.: CSC-C0502

Species: Homo sapiens (Human)

Source: Perineus; Muscle

Morphology: large transparent epitheloid cells, partially with long extensions, growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0502

Description

Cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation

Species

Homo sapiens (Human)

Source

Perineus; Muscle

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

large transparent epitheloid cells, partially with long extensions, growing as monolayers

Karyotype

Human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q)

Disease

Alveolar Rhabdomyosarcoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin +, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.