Established from the liver of a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being differentiation-responsive to erythropoietin (EPO) and unresponsive to DSMO
Murine near-diploid karyotype with 10% polyploidy
round single cells growing in suspension, some cells are slightly adherent
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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