Established from a Rauscher murine leukemia virus-induced erythroid leukemia of a DBA/2 mouse; in contrast to RED-1, cells were described to be unresponsive to erythropoietin (EPO) or DMSO
Murine near diploid karyotype with 12.5% polyploidy - 39-42XX, Df(2E2F1) - 1-2 centric fusion markers present - resembles published karyotype
round, single cells growing in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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