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RCH-ACV

  • Specification
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Cat.No.
CSC-C0556
Description
Established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy
Species
Human
Source
B cell precursor leukemia
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Culture Properties
suspension
Morphology
small round cells growing singly or in small clumps in suspension; occasional adherent cells present
Karyotype
Human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
How do you isolate cells from complex biological samples?
A:

The most common cell separation techniques include immunomagnetic cell separation, fluorescence-activated cell sorting (FACS), density gradient centrifugation, immunogenicity cell isolation, and microfluidic cell sorting.

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Average Rating: 4.0    |    1 Scientist has reviewed this product

Reliable results

The ability of this company's cell products to remain free of contamination during transport, storage and experimentation is important for obtaining reliable results.

10 May 2023


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