KYSE-410

Specification
Recommended Products

Cat.No.

CSC-C0429

Description

Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from the cervical esophagus of a 51-year-old Japanese man prior to treatment (tumor invasion into the adventitia was obvious); described to overexpress hst-1 (= heparin-binding growth factor) and cyclin D1

Species

Human

Source

esophageal squamous cell carcinoma

Recommended Medium

90-95% RPMI-1640 + 5-10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid adherent cells growing in monolayers

Karyotype

Human flat-moded hypotetraploid karyotype with 4% polyploidy - 79-91<4n>Xder(X), -10, -18, -19, -21, -22, -22, +6mar, add(X)(qter), add(1)(p11)/del(1)(p13), add(3)(q21)x1-2, add(5)(p13), der(6)t(4;6)(q12;q14)x1-2, dup(7)(p12p14), der(7)del(7)(p12p21)t(7;9

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.