Established from the pleural effusion of a 60-year-old Asian man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a P53 mutation (C455T)
epithelial cells growing adherently as monolayer and small clusters
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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