KYAE-1

Cat.No.: CSC-C0683

Species: Homo sapiens (Human)

Source: Pleural Effusion Metastasis

Morphology: epithelial cells growing adherently as monolayer and small clusters

Culture Properties: monolayer

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Cat.No.

CSC-C0683

Description

Established from the pleural effusion of a 60-year-old Asian man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a P53 mutation (C455T)

Species

Homo sapiens (Human)

Source

Pleural Effusion Metastasis

Recommended Medium

40%RPMI1640 + 40%Ham's F-12 + 20%h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial cells growing adherently as monolayer and small clusters

Disease

Esophageal Adenocarcinoma

Quality Control

Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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