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KCI-MOH1

  • Specification
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Cat.No.
CSC-C0509
Description
Originally described to be derived from the moderately differentiated adenocarcinoma of the head of the pancreas from a 74-year-old African-American man in 1996; patient was treated with pancreato-duodenectomy; cells were described as being tumorigenic in SCID mice; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line HPAC which was established in 1985 from the pancreas adenocarcinoma of a 64-year-old Caucasian woman
Species
Human
Source
pancreas adenocarcinoma
Recommended Medium
Culture Properties
monolayer
Morphology
adherent epithelial-like cells growing in monolayers
Karyotype
Human hypotriploid karyotype with 8% polyploidy - 61(57-63)<3n>X/XX, -Y, +1, -2, -6, +8, -10, +11, -12, -13, -14, -16, -18, -21, -21, +mar, add(8)(p2 ?)x2, i(11p), del(11)(p12), add(13)(p11), add(17)(p12) - differs from published karyotype
Quality Control
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endoth
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
Are there any specific culture conditions required for adherent cells?
A:

Adherent cells often require a solid surface or substrate for attachment, which is typically a tissue culture-treated plastic surface. They also require a suitable culture medium that provides the necessary nutrients, growth factors, and hormones for cell growth and proliferation.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Stable growth

The growth of these tumor cell products was stable, which proved to be crucial in our study.

11 Dec 2022


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