Originally described to be derived from the moderately differentiated adenocarcinoma of the head of the pancreas from a 74-year-old African-American man in 1996; patient was treated with pancreato-duodenectomy; cells were described as being tumorigenic in SCID mice; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line HPAC which was established in 1985 from the pancreas adenocarcinoma of a 64-year-old Caucasian woman
Human hypotriploid karyotype with 8% polyploidy - 61(57-63)<3n>X/XX, -Y, +1, -2, -6, +8, -10, +11, -12, -13, -14, -16, -18, -21, -21, +mar, add(8)(p2 ?)x2, i(11p), del(11)(p12), add(13)(p11), add(17)(p12) - differs from published karyotype
adherent epithelial-like cells growing in monolayers
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endoth
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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