KCI-MOH1

Cat.No.: CSC-C0509

Species: Human

Source: pancreas adenocarcinoma

Morphology: adherent epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0509

Description

Originally described to be derived from the moderately differentiated adenocarcinoma of the head of the pancreas from a 74-year-old African-American man in 1996; patient was treated with pancreato-duodenectomy; cells were described as being tumorigenic in SCID mice; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line HPAC which was established in 1985 from the pancreas adenocarcinoma of a 64-year-old Caucasian woman

Species

Human

Source

pancreas adenocarcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing in monolayers

Karyotype

Human hypotriploid karyotype with 8% polyploidy - 61(57-63)<3n>X/XX, -Y, +1, -2, -6, +8, -10, +11, -12, -13, -14, -16, -18, -21, -21, +mar, add(8)(p2 ?)x2, i(11p), del(11)(p12), add(13)(p11), add(17)(p12) - differs from published karyotype

Quality Control

Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endoth

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Are there any specific culture conditions required for adherent cells?

Adherent cells often require a solid surface or substrate for attachment, which is typically a tissue culture-treated plastic surface. They also require a suitable culture medium that provides the necessary nutrients, growth factors, and hormones for cell growth and proliferation.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Stable growth

The growth of these tumor cell products was stable, which proved to be crucial in our study.

11 Dec 2022

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