Fetal liver CD36+ Early Erythroid Progenitor cells are derived from FL-CD34+ cells in culture. Fetal liver-CD34+ cells are isolated from fetal liver Mononuclear Cells with a purity >90%. FL-CD34+ cells are cultured for seven to eight days using StemSpan SFEM (StemCell Technologies, Inc.), a serum free expansion media for hematopoietic cells, with the addition of EPO (5U/ml), SCF (50ng/ml), IL-3 (10ng/ml), and IL-6 (10ng/ml) growth factors. The cells are harvested from culture, and the CD36+ cells are positively isolated, using immunomagnetic CD36 MicroBeads.
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