The cells have a finite lifespan of about 25 serial passages from the tissue of origin; they can be transformed by SV40; growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the mediumTumorigenecity:Isoenzyme:G6PD, BHistopathology:normalSubculture:Split ratio:Media change:Reverse transcritase:negativeProduction
STR DNA Profile
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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