HFFF2

Cell Viability of Fe@C/Ole on HFFF2 Cells and hUC-MSCs

Skin aging, which is affected by intrinsic and extrinsic factors, leads to reduced elastin, collagen, and hydration levels. Safavizadelh et al. utilized modified exosomal content with treated Oleuropein and Fe₃O₄@C/Oleuropein to modulate gene and microRNA expression on the HFFF2 cells in vitro in order to reduce skin aging.

Fe₃O₄@C/Oleuropein was synthesized via hydrothermal method and confirmed by XRD, FTIR, and SEM. Proliferating cells from human umbilical cord explants exhibited fibroblast-like and spindle-shaped morphologies (Fig. 1A, B). The normal morphology of human dermal fibroblast cells (HFFF2) was observed using phase-contrast microscopy (Fig. 1C). Fe@C/Ole was evaluated for its effects on cell viability (Fig. 2A), with IC₅₀ values of 484.52 μg/mL for HFFF2 cells and 511 μg/mL for hUC-MSCs. Cell treatment was performed at IC₂₅ ≃ 250 μg/mL, which also showed cell growth inhibition. The effects of different Ole concentrations on HFFF2 and hUC-MSC viability were studied (Fig. 2B), with IC₅₀ values of 1035.4 μg/mL for HFFF2 cells and 977.43 μg/mL for hUC-MSCs. Cell treatment was conducted at IC₂₅ ≃ 500 μg/mL as an effective concentration. In summary, Ole exhibited cell inhibition and toxicity at very high concentrations (IC₅₀ ≃ 1000 μg/mL), while Fe@C/Ole showed these effects at lower concentrations (IC₂₅ ≃ 500 μg/mL).