Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
This DMEM Medium is modified as follows:
With: High Glucose, L-glutamine, HEPES and Phenol Red
Without: Sodium Pyruvate
2°C to 8°C (protect from light).
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