COLO-704

Cat.No.: CSC-C0323

Species: Human

Source: ovarian adenocarcinoma

Morphology: grows as clusters and single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0323

Description

Established from the ascites fluid stemming from the colon metastasis of a 46-year-old white woman with ovarian adenocarcinoma in 1986

Species

Human

Source

ovarian adenocarcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

grows as clusters and single cells in suspension

Karyotype

Human hyperdiploid karyotype with 22% polyploidy - 51(48-53)<2n>XX, +1, +7, +8, +8, -17, +19, +20, i(1q), der(8)t(8;?)(q21;?) - dm present in 16% cells - i(1q) is associated with ovarian carcinoma - identification confirmed

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Virus

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How are cell culture models of ovarian cancer created?

Cell culture models of ovarian cancer are created by isolating cancer cells from patient samples or established cell lines and growing them in a suitable culture medium.

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