Established from the ascites fluid stemming from the colon metastasis of a 46-year-old white woman with ovarian adenocarcinoma in 1986
grows as clusters and single cells in suspension
Human hyperdiploid karyotype with 22% polyploidy - 51(48-53)<2n>XX, +1, +7, +8, +8, -17, +19, +20, i(1q), der(8)t(8;?)(q21;?) - dm present in 16% cells - i(1q) is associated with ovarian carcinoma - identification confirmed
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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