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COLO-704
Cat.No.
CSC-C0323
Description
Established from the ascites fluid stemming from the colon metastasis of a 46-year-old white woman with ovarian adenocarcinoma in 1986
Organism
Human
Source
ovarian adenocarcinoma
Culture Properties
suspension
Morphology
grows as clusters and single cells in suspension
Karyotype
Human hyperdiploid karyotype with 22% polyploidy - 51(48-53)<2n>XX, +1, +7, +8, +8, -17, +19, +20, i(1q), der(8)t(8;?)(q21;?) - dm present in 16% cells - i(1q) is associated with ovarian carcinoma - identification confirmed
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Virus
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Virus
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
