Bone Marrow-CD36+ Early Erythroid Progenitor cells are derived fromBone Marrow-CD34+ cells in culture. The Bone Marrow-CD34+ cells are cultured for 7 to 8 days using StemSpan SFEM (StemCell Technologies, Inc.), a serum free expansion media for hematopoietic cells, with the addition of SCF (50ng/ml), EPO (2U/ml), IL-3 (10ng/ml), and IL-6 (10ng/ml) growth factors. The cells are harvested from culture, and the Bone Marrow-CD36+ cells are positively isolated, using direct immunomagnetic CD36 MicroBeads labeling system
Storage and Shipping
Store in liquid nitrogen and ship in dry ice.
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