Description
Murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6
Recommended Medium
15% + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine
Culture Properties
suspension
Morphology
round cells, growing single or clustered in suspension
Karyotype
Murine karyotype
Quality Control
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell di
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^5 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-208-144-6005
