Murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6
15% + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine
round cells, growing single or clustered in suspension
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell di
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^5 cells/ampoule; ship in dry ice; store in liquid nitrogen
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