K562/Adr

Effect of Low-Dose Radiation on the Kinetics of Pirarubicin and Daunorubicin Transport in K562 Cells and Drug Resistant K562/adr Cells

Low-dose radiation may affect drug transport kinetics. Here, Supawat et al. irradiated K562 and K562/adr cells with 137Cs gamma radiation at doses of 0, 0.02, 0.05, and 0.1 mGy and assessed cell viability and Pira/Dau transport kinetics at 0, 1, 4, and 24 hours post-irradiation.

Figure 1 A-F show the OD at 570 nm values in K562 and K562/adr cells collected at 0, 1, 4, and 24 h after in vitro exposure to various low doses of gamma radiation, followed by treatment with Pira and Dau. The OD at 570 nm indicates cell viability. At 0 h post-irradiation, significant changes in OD at 570 nm were observed in 0.1 mGy-irradiated K562/adr cells compared to non-irradiated K562/adr cells (Fig. 1D). Significant changes in OD at 570 nm were seen in irradiated K562/adr cells at 24 h post-irradiation compared to 0 h post-irradiation, while no significant changes were observed at 1 and 4 h post-irradiation (Fig. 1D). Significant changes in OD at 570 nm were also observed at 1, 4, and 24 h post-irradiation in 0.02 mGy-irradiated K562/adr cells treated with Pira compared to 0 h post-irradiation. Similarly, significant changes were seen at 24 h post-irradiation in 0.05 and 0.1 mGy-irradiated K562/adr cells treated with Pira compared to 0 h post-irradiation (Fig. 1E). At 0 h post-irradiation, significant changes in OD at 570 nm were observed in 0.1 mGy-irradiated K562/adr cells treated with Dau compared to non-irradiated K562 cells treated with Dau. Significant changes in OD at 570 nm were also seen at 24 h post-irradiation in 0.05 and 0.1 mGy-irradiated K562/adr cells treated with Dau compared to 0 h post-irradiation (Fig. 1F).