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Immortalized Mouse Progenitor-Like Melanocytes (iMC23)
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Cat.No.
CSC-I9233L
Description
Epidermal melanocytes are melanin-producing cells that function as a protective mechanism from harmful UV rays. Melanoma has been associated to defects, dysfunction, or lack of melanocytes. Primary C57BL/6 mouse melanocytes have been derived and successfully immortalized with SV40 large T antigen. The Immortalized Mouse Progenitor-Like Melanocytes (iMC23) are hygromycin-resistant cells which expresses melanogenic markers (c-kit, Pax3, Sox10, and MITF-m), and are non-tumorigenic. By culturing the cells in the presence of dexamethasone, differentiation occurs and yields mature melanocytes expressing HMB45 and has low tyrosinase activity. These cells are a valuable tool in melanocyte biology and molecular pathogenesis of pigment-related cell disorder studies.
Species
Mus musculus
Source
Skin
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and transduction with retroviruses carrying SV40 T antigen (SV40T) flanked with FRT sites
Markers
c-kit, Pax3, Sox10, MITF-m
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Fontana-Masson staining for melanocytic cell markers;
2) Tyrosine enzymatic assays determined presence of tyrosine;
3) Western blot analysis for SV40 large T antigen presence
2) Tyrosine enzymatic assays determined presence of tyrosine;
3) Western blot analysis for SV40 large T antigen presence
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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