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Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT)
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Cat.No.
CSC-I9358L
Description
The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) were derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of control mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. These cells provide solution in difficulty in maintaining mouse aortic endothelial cells in multiple passages without phenotypic drift.
Species
Mus musculus
Source
Heart
Culture Properties
Adherent
Morphology
Elongated
Immortalization Method
Immortalization using polyoma middle-sized T-antigen (PmT)
Markers
PECAM1, eNOS, VE-cadherin, and von Willebrand Factor
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CSC-C1861 C57BL/6 Mouse Aortic Endothelial Cells
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) FACS cell sorting;
2) Characterization by Dil-Ac-LDL staining and immunostaining
2) Characterization by Dil-Ac-LDL staining and immunostaining
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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