Human iPSC-derived Keratinocytes

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Background
Scientific Data
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Cat.No.

CSC-00840L

Description

Human iPSC-derived Keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, genetic-elements-free, virus-free, induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Human iPSC-derived Keratinocytes are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratin-18. Human iPSC-derived Keratinocytes can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.

Species

Human

Culture Properties

Adherent

Application

For Research Use Only

Shipping

Dry Ice

Quality Control

Sterility, Safety, (BioSafety Level 2). HIV/viruses, bacteria, fungi: negative, Cell viability post-thawing >85%

Storage and Shipping

Remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Human induced pluripotent stem cell (iPSC)-derived keratinocytes represent a revolutionary alternative to primary human keratinocytes for skin biology, disease modeling, and regenerative medicine. Compared to primary keratinocytes, iPSC-derived counterparts exhibit superior advantages: (1) unlimited self-renewal - they circumvent the limited proliferative capacity and donor-to-donor variability of primary cells; (2) genetic identity preservation - patient-derived lines enable autologous grafts and personalized disease modeling (e.g., epidermolysis bullosa, ichthyosis); (3) scalable and reproducible manufacturing - freeze-thaw stable, standardized batches reduce experimental inconsistencies; (4) ethical compliance - no need for cadaveric or surgical skin biopsies. Moreover, CRISPR-corrected iPSC-derived keratinocytes hold therapeutic promise for genodermatoses. Thus, this innovative platform accelerates drug screening, toxicity testing, and skin tissue engineering, outperforming conventional sources in both research and translational applications.

iPSC-Derived Reconstructed Epidermal Skin Model as an Alternative Model for Skin Irritation

The limited availability of primary normal human epidermal keratinocyte (NHEK) has hampered the large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Therefore, in this study, we developed a reconstructed epidermal skin model using the iPSC-derived keratinocytes (iPSC-derived-RHE). The generated skin model exhibited a multilayered epidermis with a distinct cornified layer at the surface, similar to human epidermis. This indicates that the iPSC-derived keratinocytes had differentiated and organized into a functional epidermal tissue.

Several test chemicals with known irritation potentials were applied to the surface of the reconstructed skin model for skin irritation tests. The MTT assay was used to evaluate the viability of the keratinocytes after exposure to the test chemicals. The results show that the iPSC-derived-RHE predicted skin irritations in a manner similar to the commercially available SkinEthic system.

These findings demonstrate the successful development of a reconstructed epidermal skin model using iPSC-derived keratinocytes, which can be used for skin irritation testing and cosmetic research.

Reconstructed epidermal skin model and immunofluorescence staining. — Fig. 1. Gene expression and immunofluorescence staining results of iPSC-derived RHE and RHE (Xie, Tong, *et al.*, 2025).

Skin irritation tests of iPSC-derived-RHE compared to commercially available SkinEthic systems. — Fig. 2. iPSC-derived-RHE were compared to commercially available SkinEthic systems for skin irritation testing (Xie, Tong, *et al.*, 2025).

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For research use only. Not for any other purpose.