Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium.
This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.
To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit. The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.
To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal or Primary Epidermal Melanocytes, Normal, Human Adult), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit or the Adult Melanocyte Growth Kit.
Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.
Optional media supplements:
Gentamicin-Amphotericin B Solution
Penicillin-Streptomycin-Amphotericin B Solution
Cell testing: Rate of proliferation and morphology.
pH: 7.5 ± 0.2
Osmolality: 315 ± 10 mOsm
Endotoxin: < 0.5 EU/mL
Sterility Testing: Negative for bacteria, fungi and yeast.
Storage and Shipping
2 to 8 °C, protect from light
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