SET-2

Cat.No.: CSC-C0608

Species: Human

Source: essential thrombocythemia

Morphology: mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells

Culture Properties: suspension

Datasheet

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0608

Description

Established from the peripheral blood of a 71-year-old woman with essential thrombocythemia at megakaryoblastic leukemic transformation in 1995; cells were described to carry the JAK2 V617F mutation

Species

Human

Source

essential thrombocythemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells

Karyotype

Human flat-moded near-diploid karyotype with 8% polyploidy; 46-47<2n>XX, +13, -14, +21, +mar, add(4)(p15), der(5)t(5;19)(q11;q13), der(7)t(7;14)(q31;q11), del(9)(p11p24)ins(9;?21)(p11;p11p12), add(9)(q11)t(9;22)(p24;q12), der(13)del(13)(q11-q13)t(13;21)(q

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD38 +, CD42 -, CD71 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the limitations of karyotyping?

Conventional karyotyping is limited by its inability to identify cryptic abnormalities, complex aberrations, and marker chromosomes accurately.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

New insights

We have been able to use this product to study the effects of radiation on cancer cells, leading to new insights.

11 Mar 2023

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

MeMo 05

Description: Species: human male 66 years old; Tissue: lymph node, metastasis; Tumor: melanoma

Cat#: CSC-6296W INQUIRY

View Product

Daudi

Description: Species: human - male, 16 years old, BlackReceptor: complement; Fc of Ig GTumorigenecity: yes, , in nude mice; form colonies in agaroseIsoenzyme: G6PD, BKaryology: normal male; diploid; ...

Cat#: CSC-C9369L INQUIRY

View Product

REH

Description: Species: human (Homo sapiens)Cell type: B cell precursor leukemiaOrigin: established from the peripheral blood of a 15-year-old North African girl with acute lymphoblastic leukemia (ALL at first ...

Cat#: CSC-C0205 INQUIRY

View Product

ROV-S

Description: Species: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr; Derived from: children with maturation defects of haematopoiesis

Cat#: CSC-6310W INQUIRY

View Product

SCC-3

Description: Human monocytic cell line expressing Interleukin-2 receptor.

Cat#: CSC-C6668J INQUIRY

View Product

UT-7

Description: established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7); cells are constitutively cytokine-dependent and responsive to various cytokines

Cat#: CSC-C0294 INQUIRY

View Product

For research use only. Not for any other purpose.