Description
Established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at Creative Bioarray by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR
Karyotype
Human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype
Source
peripheral blood B lymphoblastoid cells
Culture Properties
suspension
Morphology
ovoid to round cells growing in suspension, singly or in clumps
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transc
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
