Established from the peripheral blood of a 64-year-old man with acute monocytic leukemia (AML FAB M5) at relapse in 1985 following myeloid metaplasia; simultaneous sister cell line of MONO-MAC-6 (DSM ACC 124); cells are described to be less differentiated than MONO-MAC-6 and to support productive infection with HIV-1
acute monocytic leukemia
single, round/multiformed cells in suspension
Human near-diploid karyotype with 10% polyploidy - 49(43-52)<2n>XY, +8, +12, +13, +18, -20, inv dup(3)(q21q27), add(8)(q24), t(9;11)(p22;q23), t(10;12;17)(q24;q13;q11), r(12)(??), del(13)(q1?2q21), t(16;21)(q13;q22.2) - diploid version of its sister MONO-
A new in vitro model system to study HIV-1 infection in human cells of the mononuclear phagocyte series.
Frozen with 70% medium, 20% FBS, 10% DMSO;
Ship in dry ice; Store in liquid nitrogen
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD34 -, cyCD68 +, HLA-DR +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml
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