Established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) in 1986
Human flat-moded aneuploid hypertriploid karyotype - 79(72-79)<3n>XX, -X,-1, +3, +4, +5, +6, +8, +11, +12, -13, +14, +15, +16, +18, -19, +20, +21, +22, +dminx1-4, add(1)(p11-21), del(3)(q22), der(4)add(4)(p1?)t(4;11)(11;?)(q33;q13)(q24;?)x2, der(5)t(1;5)(
single, elongated, snake-like cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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