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F-36P

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Cat.No.
CSC-C0551
Description
Established at diagnosis from the pleural effusion of a 68-year-old man with acute myeloid leukemia (AML M6) secondary to myelodysplastic syndrome (MDS, subtype refractory anemia with excess of blasts, RAEB) in 1989; cells were described to be proliferatively responsive to GM-CSF and IL-3
Organism
Human
Karyotype
Human flat-moded hypodiploid karyotype with 6% polyploidy - 41(40-43)<2n>XY, -3, -13, -16, -19, -21, -22, +2mar, ins(X;13)(p11;q?q?), del(3)(p13p24), add(5)(q11), dup(5)(q11q35), der(6)add(6)(p23)dup(6)(q25q27), der(7)add(7)(p13)add(7)(q12), add(9)(q35),
Source
acute myeloid leukemia
Morphology
polymorph cells growing as adherent cells and singly in suspension
Recommended Medium
80% RPMI-1640 + 20%h.i. FBS + 10 ng/ml GM-CSF
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 -, CD42 -, CD71 +, CD235a +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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