Established in 1974 from a bone marrow metastasis of a 10-month-old girl with neuroblastoma; cells are described to express an opiate binding site
polymorphic cells (mostly epithelial with short neurites) growing adherently in monolayers
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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