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HCEC-H9C1

Cat.No.: CSC-C6214X

Species: Human

Source: corneal endothelium

Morphology: polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers

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Cat.No.
CSC-C6214X
Description
HCEC-H9C1 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 established from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described to represent developing or transitional cells of the so-called corneal endothelium
Species
Human
Source
corneal endothelium
Recommended Medium
Human-Endothelial-SFM + 10 ng/ml FGF-2 (do not apply h.i. FBS in order to preserve differentiation status)
Morphology
polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 (+), cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How are microscopy and imaging techniques incorporated into quality control in cell biology?

Microscopy and imaging techniques are used to monitor cell morphology, assess cell proliferation and behavior, and confirm the absence of microbial contamination in cell cultures.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Highly recommend

As a researcher, I highly recommend this tumor cell product for its consistency and reliability.

15 Mar 2023


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