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HCEC-H9C1

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Cat.No.
CSC-C6214X
Description
HCEC-H9C1 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 established from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described to represent developing or transitional cells of the so-called corneal endothelium
Organism
Human
Source
corneal endothelium
Morphology
polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers
Recommended Medium
Human-Endothelial-SFM + 10 ng/ml FGF-2 (do not apply h.i. FBS in order to preserve differentiation status)
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 (+), cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6321W
CSC-C0316
CSC-C0351
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