HCEC-H9C1
Cat.No.: CSC-C6214X
Species: Homo sapiens (Human)
Source: Eye; Cornea
Morphology: polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers
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Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 (+), cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,
HCEC-H9C1 is an immortalized human corneal epithelial cell line which was developed from primary human corneal epithelial cells and immortalized by regulated genetic modification so that the cell line can proliferate indefinitely. Characteristics of this cell line include epithelial morphology (polygonal shape), expression of cytokeratins such as CK3/12 and formation of cell-cell junctions characteristic of barrier function. While retaining characteristics important to epithelial morphology and function, HCEC-H9C1 cells provide an alternative to primary cells with greater stability and reproducibility in culture.
HCEC-H9C1 cells are frequently used as a model of corneal epithelial function. This includes investigation into epithelial proliferation and differentiation as well as wound healing and regulation of corneal barrier function. Cells can be stimulated with inflammatory cytokines, environmental stresses or microbes to study mechanisms of disease on the ocular surface such as dry eye or infection and cellular damage. They can also be used to assess the safety and bioactivity of drugs, biomaterials and drug formulations intended for ocular use.
Oxybuprocaine Inhibits the Proliferation of HCEC-12, HCEC-H9C1, and HCEC-B4G12
Oxybuprocaine is routinely used clinically as an eye anesthetic drug. To evaluate the effects of oxybuprocaine on various types of human corneal endothelial cells, HCEC-12, HCEC-H9C1 and HCEC-B4G12 were induced by oxybuprocaine media with different concentrations for 0, 3, 6, 12 and 24 h, and Cell Counting Kit-8 (CCK-8) assay was used to detect the activity of HCEC-12, HCEC-H9C1 and HCEC-B4G12 after reaction with oxybuprocaine. The results showed that the activity of HCEC-12, HCEC-H9C1 and HCEC-B4G12 was inhibited, and this effect was dose-dependent; furthermore, the inhibition of cell activity was significantly enhanced in HCEC-12 compared with that in HCEC-H9C1 and HCEC-B4G12 after incubation with oxybuprocaine concentrations greater than 100 mg/l for 12 h (P<0.05) (Fig. 1A-C). Therefore, they selected HCEC12 for subsequent experiments.
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