CAL-120

Cat.No.: CSC-C0488

Species: Human

Source: breast adenocarcinoma

Morphology: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0488

Description

Established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991

Species

Human

Source

breast adenocarcinoma

Recommended Medium

90% DMEM+ 10% h.i.FBS

Culture Properties

monolayer

Morphology

heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers

Karyotype

Human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), de

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I do in case of cross-contamination of cells during cell culture?

If cross-contamination is suspected, cytogenetic identification can be done.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good product

I have purchased CAL-120 products for scientific research and recommended it to others.

15 Sep 2021

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For research use only. Not for any other purpose.