Established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells)
Human hypotriploid karyotype with 16% polyploidy - 65(57-68)<3n>XX, -X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q)
epithelial-like adherent cells growing as monolayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, HMB-45 -, neurofilame
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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