Bone marrow from SL mouse with spontaneous myeloid leukemia was injected into isogenic mice and the cell line was established from the ensuing hyperplastic lymph nodes 14 days later by recloning on soft agar.
Mus musculus, mouse
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Mouse myeloid leukemia
The M1 cell line has been used as a model for differentiation in the myeloid macrophage pathway and can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone.
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