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KYSE-70

Cat.No.: CSC-C0414

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: epitheloid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.
CSC-C0414
Description
Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria)
Species
Human
Source
esophageal squamous cell carcinoma
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently in monolayers
Karyotype
Human hypotriploid karyotype with 8% polyploidy - 60(54-63)<3n>XXX, -Y, -2, -3, -3, -4, -6, +7, -8, -9, -10, -13, -14, -15, -16, -16, -16, -17, -18, -18, -21, -22, +10mar, del(X)(q25), add(1)(p11), add(2)(q33), del(2)(q33), del(3)(q13), add(4)(q35), add(5
Quality Control
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytoker
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What treatment should be done before using the serum?

Most cell culture sera do not need to be inactivated prior to use. The purpose of inactivation is to remove the complement component of the serum and to avoid the impact of complement on subsequent experiments.

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Average Rating: 4.0    |    1 Scientist has reviewed this product

Satisfied

Generally speaking, we are very satisfied with Creative Bioarray's products and services.

23 Jan 2021


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For research use only. Not for any other purpose.