Description
Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria)
Source
esophageal squamous cell carcinoma
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently in monolayers
Karyotype
Human hypotriploid karyotype with 8% polyploidy - 60(54-63)<3n>XXX, -Y, -2, -3, -3, -4, -6, +7, -8, -9, -10, -13, -14, -15, -16, -16, -16, -17, -18, -18, -21, -22, +10mar, del(X)(q25), add(1)(p11), add(2)(q33), del(2)(q33), del(3)(q13), add(4)(q35), add(5
Quality Control
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytoker
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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