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KYSE-450

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Cat.No.
CSC-C0435
Description
Established from the well differentiated invasive eosphageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 59-year-old Japanese man prior to treatment (depth of invasion was not beyond the submucosa); described as carrying p53 mutation and amplification of the oncogene MYC
Organism
Human
Karyotype
Human hyperdiploid karyotype with 6.7% polyploidy - 57(49-57)<2n>, -Y, +1, +3, +3, +7, +12, +17, -18, +7mar, der(1)t(1;6)(p22;q11), add(1)(p32/34), del(2)(p23), del(3)(p22), del(4)(q21), add(5)(q35), del(6)(q25), add(7)(p22), add(8)(p23), add(9)(q34), del
Source
esophageal squamous cell carcinoma
Culture Properties
monolayer
Morphology
epitheloid cells growing adherently as monolayers
Recommended Medium
90%RPMI1640 or 45%RPMI1640 + 45%Ham's F-12 + 10%h.i. FBS.
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-C0314
CSC-C0404
CSC-C0405
CSC-C0414
CSC-C0419
CSC-C0422
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