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KB-V1

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Cat.No.
CSC-C6212X
Description
KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 which itself is a derivative of the 1951 established HELA (31-year-old black woman); cells express strongly mdr1 mRNA and protein; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111
Organism
Human
Karyotype
Human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)
Source
cervix carcinoma (derivative of HELA)
Culture Properties
monolayer
Morphology
epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present
Recommended Medium
85% DMEM + 15% h.i.FBS + 200-1000 ng/ml vinblastine
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, H
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6260W
CSC-6261W
CSC-6262W
CSC-6263W
Customer Support & Price Inquiry
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