KB-V1

Cat.No.: CSC-C6212X

Species: Human

Source: cervix carcinoma (derivative of HELA)

Morphology: epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present

Culture Properties: monolayer

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Cat.No.

CSC-C6212X

Description

KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 which itself is a derivative of the 1951 established HELA (31-year-old black woman); cells express strongly mdr1 mRNA and protein; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111

Species

Human

Source

cervix carcinoma (derivative of HELA)

Recommended Medium

85% DMEM + 15% h.i.FBS + 200-1000 ng/ml vinblastine

Culture Properties

monolayer

Morphology

epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present

Karyotype

Human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, H

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the current research directions in understanding lymph node metastasis?

Current research focuses on identifying molecular mechanisms of lymph node metastasis, developing imaging techniques for early detection, and exploring targeted therapies to prevent metastatic spread.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Exceptional performance

The cell biology products demonstrated exceptional performance, allowing for precise and accurate cell culture maintenance and experimentation.

02 Oct 2023

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