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COLO-818
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Cat.No.
CSC-C0237
Description
Established from malignant melanoma
Species
Human
Source
melanoma
Morphology
adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition
Karyotype
Human hypotriploid karyotype with 30% polyploidy - 62(57-63)<3n>X, -X, -X, -4, -5, +7, +8, -9, -10, -11, -13, +15, -16, -17, -19, -21, -21, +3mar, 1 dm present in 15% cells - add(3)(p11), der(13;21)(p10;10) - high level of subclonal rearrangement present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -,
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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